The translocation of 14q32 with 18q21 is the most common chromosomal abnormality in human non-Hodgkin's lymphomas. The translocation is commonly associated with the follicular lymphoma which is relatively rare in Korean. About 30% of large cell
lymphomas in Western countries have same translocation and this finding suggests that this subset of large cell lymphoma would be evolved from follicular lymphoma. Recently, the translocation breakpoint has been cloned and polymerase chain
reaction(PCR)
for detecting bcl-2/JH(immunoglobulin heavy chain gene joining segment) rearrangement, the crossover site of translocation, was introduced. Detection of bcl-2/JH rearrangement in Korean large cell lymphoma tissue should lead to better
understanding
of
the biology of lymphomas in Korea So, this study was designed to evaluate the frequency of bcl-2/Jh rearrangement in Korean non-Hodgkin's lymphomas and to compare the results with those of other countries. The DNA from total 22 cases of
non-Hodgkin's
lymphomas were examined with PCR and Southern hybridization with radiolabeled oligonucleotide probes. DNAs were extrated from 15 unfixed frozen and 7 formalin-fixed paraffin-embedded biopsy specimens. The t(14; 18) chromosomal translocation,
resulting
in bcl-2/JH fusion gene, was detected in 1(9%) of 11 diffuse large cell lymphomas and 2(14.3%) of 14 B cell lymphomas. The low frequency of bcl-2/JH rearrangement suggests different genetic consequences in lymphomagenesis of Asian. However, the
clinical
implication of bcl-2/JH rear rangement is doubtful in Korean due to quite low frequency.
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